Semrock has launched λ/2 flatness 1mm thick laser dichroic beamsplitters mounted in commercially available microscope cubes. Assembled cubes compatible with popular microscopes are now available as standard catalogue products.
Conventional microscopy cubes can compromise the flatness of the dichroic beamsplitters, thereby introducing aberrations. But super-resolution imaging systems are highly sensitive to optical wavefront distortion and demand the highest quality components for best instrument sensitivity.
Semrock’s λ/2 flatness 1mm thick laser dichroic beamsplitters minimise focus shift and aberrations in the reflected beam compared to standard products. In order to produce their full flatness potential, these filters need to be carefully mounted in microscopy cubes. Semrock has developed proprietary methods that guarantee the l/2 flatness required for super-resolution applications.
Using a 1mm thick dichroic beamsplitter in the light path minimises beam deviation, reduces light scatter in the emission path, and enables faster switching speed between different cubes. Semrock’s 1mm thick super-resolution dichroic beamsplitters are designed to minimise reflected wavefront distortion for even large diameter illumination beams and are readily available for the most popular microscopy cubes (e.g. Olympus U-MF2 & U-FF, Zeiss FL Cube EC P&C, Nikon TE 2000).