InVi SPIM microscope

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Luxendo, a Bruker company, has extended its InVi SPIM microscope to include full incubation capabilities, as well as flexible illumination and detection optics. Luxendo’s single-plane illumination microscopy (SPIM) technique reduces sampling times over conventional laser scanning confocal microscopes, while reducing phototoxicity and damaging side effects on living specimens. The new technology also enables the fastest scan speeds for volumetric imaging of small organisms, cell monolayers, and cleared tissue. InVi SPIM combines these capabilities with perfect environmental conditions and gentle sample handling to create an optimised platform for ‘pure life imaging’.

The InVi SPIM’s successful handling of extremely sensitive specimens, such as embryonic cells during their first divisions, has been proven in recent publications (Strnad et al., Nature Methods 2015; Reichmann et al., Science 2018). Other samples, such as tumour spheroids, organoids, and all kinds of culture cell lines, can now be imaged with more than 80 frames per second in full frame 2,048 x 2,048-pixel resolution up to 500 frames per second in 192 x 2,048 resolution.

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